{"product_id":"xenopus-a-laboratory-manual-9781621821809","title":"Xenopus: A Laboratory Manual","description":"Members of the frog \u003cem\u003eXenopus\u003c\/em\u003e genus are versatile model organisms used in developmental biology, cell biology, neuroscience, and toxicology research, among others. Frogs are easy to rear and maintain, and large sample sizes of embryos and later stages are readily obtained. Studies using \u003cem\u003eXenopus\u003c\/em\u003e cell-free extracts, oocytes, eggs, embryos, larval stages, and adult frogs have yielded important insights into a multitude of key biological processes from mechanisms underlying the cell cycle to embryonic development to human disease. \u003cp\u003e\u003c\/p\u003eThis new laboratory manual provides a comprehensive collection of experimental procedures for research using \u003cem\u003eXenopus\u003c\/em\u003e. It includes basic methods for \u003cem\u003eXenopus\u003c\/em\u003e husbandry, egg harvesting, embryo collection, microdissection, and visualization, as well as strategies for extract preparation, genetic manipulation, genomic analysis, transcriptomics, and proteomics. There are protocols for analyses of \u003cem\u003eXenopus\u003c\/em\u003e immunology, neurobiology, metamorphosis, and adult physiology. Toxicological studies and chemical screening methods are also included. \u003cp\u003e\u003c\/p\u003eThe manual covers both \u003cem\u003eXenopus laevis\u003c\/em\u003e and the smaller \u003cem\u003eXenopus tropicalis \u003c\/em\u003especies. It is a critical reference for laboratories that currently work with these organisms and will serve as an essential start-up guide for those seeking to start using \u003cem\u003eXenopus\u003c\/em\u003e frogs in their research for the first time.\u003cbr\u003e","brand":"Cold Spring Harbor Laboratory Press","offers":[{"title":"Default Title","offer_id":50526072078610,"sku":"9781621821809","price":41.99,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0831\/4771\/8930\/files\/img_39f135c5-98de-430e-97b5-1428ac1a8a9a.jpg?v=1731258851","url":"https:\/\/surprise-castle.myshopify.com\/products\/xenopus-a-laboratory-manual-9781621821809","provider":"Surprise Castle","version":"1.0","type":"link"}